Tuning Escherichia coli for membrane protein overexpression S Wagner, MM Klepsch, S Schlegel, A Appel, R Draheim, M Tarry, ... Proceedings of the National Academy of Sciences 105 (38), 14371-14376, 2008 | 601 | 2008 |
YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase PA Scotti, ML Urbanus, J Brunner, JWL de Gier, G von Heijne, ... The EMBO journal, 2000 | 486 | 2000 |
Consequences of membrane protein overexpression in Escherichia coli S Wagner, L Baars, AJ Ytterberg, A Klussmeier, CS Wagner, O Nord, ... Molecular & Cellular Proteomics 6 (9), 1527-1550, 2007 | 449 | 2007 |
Optimization of membrane protein overexpression and purification using GFP fusions D Drew, M Lerch, E Kunji, DJ Slotboom, JW de Gier Nature methods 3 (4), 303-313, 2006 | 404 | 2006 |
Rationalizing membrane protein overexpression S Wagner, ML Bader, D Drew, JW de Gier Trends in biotechnology 24 (8), 364-371, 2006 | 380 | 2006 |
The Escherichia coli SRP and SecB targeting pathways converge at the translocon QA Valent, PA Scotti, S High, JWL de Gier, G Von Heijne, G Lentzen, ... The EMBO journal, 1998 | 378 | 1998 |
Competition between Sec‐and TAT‐dependent protein translocation in Escherichia coli S Cristóbal, JW de Gier, H Nielsen, G von Heijne The EMBO journal, 1999 | 375 | 1999 |
BIOGENESIS OF INNER MEMBRANE PROTEINS IN ESCHERICHIA COLI J Luirink, G Heijne, E Houben, JW Gier Annu. Rev. Microbiol. 59, 329-355, 2005 | 369* | 2005 |
Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli DE Drew, G Von Heijne, P Nordlund, JWL de Gier FEBS letters 507 (2), 220-224, 2001 | 298 | 2001 |
The heme-copper oxidase family consists of three distinct types of terminal oxidases and is related to nitric oxide reductase J van der Oost, APN De Boer, JWL de Gier, WG Zumft, AH Stouthamer, ... FEMS microbiology letters 121 (1), 1-9, 1994 | 294 | 1994 |
Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis D Drew, D Sjöstrand, J Nilsson, T Urbig, C Chin, JW de Gier, ... Proceedings of the National Academy of Sciences 99 (5), 2690-2695, 2002 | 239 | 2002 |
Sec-dependent membrane protein insertion: sequential interaction of nascent FtsQ with SecY and YidC ML Urbanus, PA Scotti, L Fröderberg, A Sääf, JWL de Gier, J Brunner, ... EMBO reports 2 (6), 524-529, 2001 | 238 | 2001 |
Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor QA Valent, JWL De Gier, G Heijne, DA Kendall, CM Ten Hagen‐Jongman, ... Molecular microbiology 25 (1), 53-64, 1997 | 220 | 1997 |
Assembly and overexpression of membrane proteins in Escherichia coli D Drew, L Fröderberg, L Baars, JWL de Gier Biochimica et Biophysica Acta (BBA)-Biomembranes 1610 (1), 3-10, 2003 | 199 | 2003 |
Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21 (DE3) S Schlegel, J Löfblom, C Lee, A Hjelm, M Klepsch, M Strous, D Drew, ... Journal of molecular biology 423 (4), 648-659, 2012 | 194 | 2012 |
Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle JWL de Gier, P Mansournia, QA Valent, GJ Phillips, J Luirink, ... FEBS letters 399 (3), 307-309, 1996 | 192 | 1996 |
A scalable, GFP‐based pipeline for membrane protein overexpression screening and purification D Drew, DJ Slotboom, G Friso, T Reda, P Genevaux, M Rapp, ... Protein science 14 (8), 2011-2017, 2005 | 185 | 2005 |
Function of YidC for the insertion of M13 procoat protein inEscherichia coli: Translocation of mutants that show differences in their membrane potential dependence and sec … JC Samuelson, F Jiang, L Yi, M Chen, JW de Gier, A Kuhn, RE Dalbey Journal of Biological Chemistry 276 (37), 34847-34852, 2001 | 185 | 2001 |
YidC/Oxa1p/Alb3: evolutionarily conserved mediators of membrane protein assembly J Luirink, T Samuelsson, JW de Gier FEBS letters 501 (1), 1-5, 2001 | 185 | 2001 |
Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli JWL De Gier, PA Scotti, A Sääf, QA Valent, A Kuhn, J Luirink, ... Proceedings of the National Academy of Sciences 95 (25), 14646-14651, 1998 | 170 | 1998 |